Cell immobilization of <i>Streptomyces griseobrunneus</i> by microcrystalline cellulose for production of cyclodextrin glucanotransferase enzyme

The cyclodextrin glucanotransferase are extracellular enzymes that can hydrolysis starch as a substrate to cyclodextrin. Encapsulation and immobilization technique of microbial cells within the polymeric network have widespread applications in production enzyme. Bacterial were isolated from soil by serial dilution method. molecular identity was established using 16S rDNA sequence analysis, identified strain immobilized synthesized composites. Physicochemical characteristics composites determined field emission scanning electron microscopy, Malvern Zetasizer Nano ZS FT-IR analyses. Enzyme activity measured phenolphthalein assay. Streptomyces griseobrunneus according sequence. analyses indicated there an interaction between all elements. Scanning microscopy analysis showed cross-section without attached 1109, 948 606 U/ml alginate/chitosan/microcrystalline cellulose (MCC) sheets, agar/gelatin/MCC beads free cells, respectively. It could be concluded effective improving enzyme because its excellent properties such guarding bacterial against inhibitors toxins.